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We have shown significant repression of hepatic ERα activity in mice utilizing Ad.shERα vectors.
If not otherwise stated all mice utilized for the study were male, between the ages of 4 and 6 months.
Kim et al report their findings with a mouse model utilizing ovalbumin and Aspergillus protease to induce eosinophilic inflammation.
As an alternative, a mouse model utilizing repeated doses of bleomycin has been proposed to more closely replicate pathological features of IPF in humans (Degryse et al., 2010).
In these studies, we generated a mouse model utilizing the Cre- loxP system to ablate CENP-F protein expression in a cardiomyocyte-specific manner (termed CENP-F −/− hereafter).
The study assessed the histological effects of perineural injections of propylene glycol and three medical gels in a mouse model utilizing ultrasound guided microinjection techniques.
These cellular defects are not strictly dependent on the individual hypomorphic MCM allele used, as a mouse model utilizing an MCM2IRES-CreERT2/IRES-CreERT2 allele, which resulted in a reduction in total protein level to roughly 1/3 the wild type amount, exhibited similar defects in dormant origin usage and genome instability [45,62].
To gain insights into EpCAM function in vivo, we generated EpCAM −/− mice utilizing an embryonic stem cell line with a tacstd1 allele that had been disrupted.
Consistent with the mRNA data, we further confirmed the trend that protein expression of Hif1α was upregulated in the pancreas of pVHL deficient mice utilizing a Hif1α ELISA assay (Figure 6B).
TLR2−/− mice were derived onto the 129S6 background by iterative backcrossing of B6.TLR2−/− with 129S6.RAG2−/− mice utilizing a marker-assisted, "speed congenic" breeding method 62, 63.
PS and PCS models were developed in mice utilizing a communication box according to the method of Ogawa and Kuwabara [ 25].
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