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Kaede expression in these animals was restricted to ocular muscles, compared to a more widespread expression in non-injected specimens.
This pattern is reminiscent of that observed by Caubet [42], where a c-fos exon 4 probe, revealed a more widespread expression in spinal cord and brain of E14 embryos than we found.
In a similar fashion, NEUROD was also found to be present in a discrete layer above the VZ, while TBR1 exhibited a more widespread expression pattern including the SVZ and CP (Fig. 3A C).
While NEFM is highly enriched in the nervous system, the other two have a more widespread expression and have been detected in various cells and tissues at both RNA and protein level.
The transcriptional regulation of Cyp11a1 in steroidogenic tissues is complex and involves the concerted action of several tissue-specific trans-regulators such as SF-1, DAX1, TReP-132, LBP, and GATA together with transcription factors having a more widespread expression, including AP-1, Sp1, and AP-2 [ 16].
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Given synapsin is a vesicular protein found at the synapse, the more widespread expression of neurexin I suggests it is expressed beyond the synapse.
It has been reported that GAT2 and GAT3 are expressed in the rat taste system, with GAT3 expressed primarily at the margin of the taste bud and in some TRCs, while GAT2 had more widespread expression [43].
Our results confirm expression of FGF8 in the ovary, however more widespread expression was apparent.
CBX1A and DNMT3AA showed more widespread expression in the developing brain.
CcCPI-2 and CcCPI-3, having lower and more widespread expression, could be more general "house-keeping" CPI genes.
The e1 isoform is much more abundant in the brain and also demonstrates more widespread expression throughout development.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com