More stringent experiments would have been to determine this question in an osk166 allele, a missense mutation (rather than the 84 nonsense allele) that affects germ plasm assembly, or other mutations downstream of Oskar that act in the germ plasm assembly pathway (Vasa, Tudor).
Each new published experiment sets a more stringent limit on the decay lifetime that helps theorists rule out various models and guides experimentalists on where to look next.
Thus, this perturbation experiment is a more stringent test of the model since it is required to accurately predict the combination of control as well as perturbation conditions within the same simulation.
Interaction of MYBR1 and PYL8 was confirmed by three independent experiments using a more stringent screening of positive clones on four drop out media (SD/-Leu/-Trp/-His/-Ade) in the presence of antibiotic Aureobasidin A. Furthermore, we also fused the full length MYBR2 to the BD-domain and found that it also interacts only with PYL8 out of the 14 PYL family members.
To account for this potential issue and ensure that task-relevant responses can genuinely be triggered during sleep, we implemented a more stringent control of vigilance in the second experiment, where only NREM2 brain activity was considered in the sleep condition.
To enforce a more stringent distance constraint in the cysteine crosslinking experiments, we used bis-maleimidoethane (BMOE), the shortest available bis-maleimide crosslinker (∼8 Å).
In particular, drug sensitivity testing on primary cells is often based on dose response experiments, which pose a more stringent requirement for data quality and for intra- and inter-plate variation.
Experiment 2 replicated these findings with a more stringent method designed to rule out more parsimonious explanations.
Based on the current results we expect that an even more reliable parameter determination and a more stringent verification of the simulation model could be performed with a set of experiments specifically designed for reproducible, quantitative measurements.
For this experiment, a lower concentration of DHA (10 uM) was used as a more stringent test for increased cytotoxicity.
In order to identify the most significantly influenced pathways in vivo, a more stringent p value (p < 0.002) was applied, which resulted in 1106 significantly regulated transcripts for the in vivo TNT experiment.
