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As a more sensitive procedure, HMM could overestimate the functional gene numbers without manual curation.
The expression of UBB+1 in brain sections of UBB+1 tg mice was examined using the UBB+1-specific Ubi3 antibody (bleeding 16-9-97) and a more sensitive procedure (avidin biotin method) than previously used [ 11].
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Probably, the use of more sensitive procedures such as PCR, currently available, would have increased these frequencies.
Functional IRES elements have been proposed in several eukaryotic genes, but subsequent studies using more sensitive procedures have questioned the validity of several of them [ 29- 31].
Thus, it is crucial to develop a more sensitive and reliable procedure that requires less RNA.
These data combined indicate IS-RT-PCR may serve as a more sensitive, one-step procedure for the examination of MMP gene expression in human breast cancers.
Sentinel lymph node biopsy (SLNB) is a more sensitive and accurate nodal staging procedure than axillary lymph node dissection (ALND).
However, a 24-h exposure to either VEGF or HGF induced a significant increase in VEGFR2 phosphorylation, as detected by anti-phospho-tyrosine IB analysis of VEGFR2 IP (Fig. 4A), or a more sensitive sandwiched-ELISA technique (see Supplemental Experimental Procedures) (Fig. 4B and C).
Thus, we conclude that flow cytometry is a more sensitive assay for the quantification of apoptosis than the In Cell Western procedure described here.
Privacy of VRUs (e.g. children) is a more sensitive matter than vehicle related privacy, and hence the establishment of standardised procedures that guarantee the privacy of VRUs and the security of the messages is a critical issue.
Furthermore, FPNI-PCR represents a more sensitive, rapid and accurate technique than the established TAIL-PCR and hiTAIL-PCR procedures.
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