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In contrast, the second modification, which combines ChIP with specialized CpG microarrays, is ideal for a more global analysis of target genes.
To establish a more global analysis of muscle phenotype we subjected the muscle samples to Affymetrix gene array analysis followed by our comparative array analysis strategy [30], [31].
However, they compared GEP with chromosome 3 FISH rather than a more global analysis evaluating chromosome 8 imbalances such as that provided by MLPA or array-CGH.
Future studies will have to try a more global analysis of this network as opposed to focusing on a limited number of readouts.
However, the strong coregulation in the abundance of lipid species identified in this study necessitated a more global analysis of potential lipid function.
In this study, we therefore used a pharmacological expression reactivation approach, to allow a more global analysis of gene expression changes using expression microarrays.
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This was twice as high as that after one hour without stimulation (PI = 0.05), but may well have gone undetected in their more global analysis.
More global analysis of gene expression in banana has been restricted to subtractive hybridisation and PAGE-DDGE, both of which fail to give a comprehensive picture of the transcriptome.
Thus, the main purpose of the present study was: a) to evaluate the effect that the lack of AQP4 has on DGC and ECM expression and localization; and b) to conduct a more global protein analysis to assess altered protein patterns in skeletal muscle of AQP4-null mice.
The next logical step would be to consider a more global sensitivity analysis by investigating combinations of parameters, which was beyond the scope of this study.
Application of a citrus cDNA microarray has suggested that unique genetic regulatory networks arise during fruit development [ 10], while a more global transcriptome analysis was able to identify ethylene-responsive genes in the mandarin fruit [ 11].
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