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This was assessed by performing the positional correlation of the plus and minus tags from the sequencing of fragmented but non-immunoprecipitated genomic DNA, which yielded a more diffuse profile with a maximum at 180 bp, suggesting that chromatin sonication resulted mostly in mononucleosomal particles (see Additional file 1: Figure S9).
Jarid1b occupancy (∼15-fold compared with untreated controls) was detected at the −3.1- to −2.2-kb region, culminating at −2.7 kb position of the CpG island, matching thus the positional occupancy patterns of Egr2 and of decreased H3K4 methylation, while Jarid1a occupancy exhibited a more diffuse profile.
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Genes with dense 5' methylation dominate this profile and a more diffuse pattern emerges when these genes are excluded (Supporting Information S1).
A similarly analyzed RNA Polymerase II profile showed a more diffuse and tailing distribution, which might reflect the progression of the polymerase along transcribed genes.
"When you have a high-profile company doing anything, that tends to get into the mind of the culture, and it can have a more diffuse effect".
But broadcast ads tend to reach a more diffuse mass audience with potentially less appeal to the D.S.L. marketers.
Mesh networks reconfigure the typical hub-and-spoke model of the internet into a more diffuse method of internet access.
Axial (a) and coronal reformatted (b) HRCT images show a more diffuse pattern of crazy-paving.
Contrast enhancement may vary from peripheral rim enhancement to a more diffuse pattern.
A positive staining is also present in cytoplasm but in a more diffuse pattern.
Such distribution was not apparent in cardiomyocytes that displayed a more diffuse CK1δ staining (Figure 5B).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com