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To reduce variability in the phenotype analysis, a more defined genetic background was obtained by backcrossing Ugt1 mutant mice for more than nine generations to wild-type (WT) C57BL/6 mice, reaching at least 99.8% of C57BL/6 genetic background.
On DIV 11, culture medium was replaced by low-serum Nb-N2 medium (Neurobasal medium, 0.5% heat-inactivated horse serum, 2 mM glutamine, 50 units per ml penicillin, 50 μg/ml streptomycin, 0.6% glucose, 1 × N2 supplement, pH 7.2) to ensure more defined condition during analysis.
His "salute" was a lot more defined.
Therefore we conducted a second level BAPS analysis which split the current five BAPS populations into more defined populations; however the second level BAPS analysis still did not explain the population structure (Additional file 2 and Additional file 8: Figure S6).
ActinEGFP-positive spines are much more defined than those expressing EGFP, facilitating analysis.
"More defined. . .
"You look more defined".
Mathematics is not a substitute for judgment, nor do equations define analysis.
Nevertheless, we performed an additional analysis on a more strictly defined sexual gene category only including genes with Q < 0.10.
We repeated the latter analysis in a more strictly defined group (N = 44) of cases suffering from sepsis or severe bacteraemia and their matched controls.
I could no more define poetry than a terrier can define a rat (A. E. Housman).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com