The phrase "a module vector" is correct and usable in written English.
It can be used in contexts related to mathematics, programming, or engineering, where it refers to a vector associated with a module.
Example: "In our analysis, we will consider a module vector that represents the system's state."
Alternatives: "a vector module" or "a vector associated with a module".
We developed a CRISPR/Cas9-based binary vector set and a gRNA module vector set as a toolkit for multiplex genome editing in plants.
Incorporation of DESTination cassettes (Invitrogen) allows essentially any plasmid to be converted into a ligation-free module vector to allow for facile transfer of any gene into expression plasmids using the simple Gateway LR technology (Invitrogen).
We developed a CRISPR/Cas9 binary vector set based on the pGreen or pCAMBIA backbone, as well as a gRNA (guide RNA) module vector set, as a toolkit for multiplex genome editing in plants.
Analysis of this limited data set using a phylogenetic tree (AlignX module, Vector NTI Suite 7.0, Informax, Bethesda, MD) produced no distinct groupings of cases separate from the healthy subjects (data not shown).
Any module vector having a small angle to the perpendicular line (the red line in Fig. 1) cannot effectively distinguish the two conditions, meaning that similar expression levels (i.e., the projection lengths) on the gene module have been observed in the two conditions.
Thus, the binary vector set combined with the gRNA module vector set comprise an efficient, inexpensive, time-saving, user-friendly, multifaceted, extensible toolkit for the generation of CRISPR/Cas9 binary vectors carrying one or more gRNAs for targeted mutations of multiple genes.
The binary vector set combined with the gRNA module vector set comprise an efficient, inexpensive, time-saving, user-friendly, multifaceted, extensible toolkit for the generation of CRISPR/Cas9 binary vectors carrying one or more gRNAs for targeted mutations of multiple plant genes.
Therefore, we claim that the gene module is specific for a condition if it fulfills two criteria: (1) the length of the gene module vector projected on the condition vector is relatively long, and (2) the gene module vector is almost perpendicular to the connected line (e.g., the blue line in Fig. 1) between any other two conditions.
Therefore, the gRNA module vector set is extensible and can easily be updated.
All binary vectors were transformed into Agrobacterium tumefaciens strain UIA 143. cDNA of the glycoproteins was cloned into the magnICON TMV-based module vector (TMV3': pICH21595, Bayer BioScience NV Research, Ghent, Belgium) containing two BsaI sites designed for directional cloning of the target gene (Marillonnet et al. 2004, 2005; Giritch et al. 2006; Figure 2).
