Exact(6)
Nitrogen-containing hollow carbon microspheres (NHCSs) are prepared by a modified template method in the presence of resorcinol/formaldehyde as carbon precursors and ethylenediamine (EDA) as both a base catalyst and nitrogen precursor.
Several enzymes were screened for the ability to incorporate these modified nucleotides, as well as to amplify a modified template.
Indeed, AMV-RT faithfully incorporates T opposite both 7-EAA and 7-EAA triazole and extends to the end of a modified template strand.
RNA-seq libraries were prepared as previously described (Kivioja et al. 2011) with the following changes: 1.8 μg of each RNA sample was used for cDNA synthesis with a modified template switch oligo (5′-ACACTCTTTCCCTACACGACGCTCTTCCGATCT- 5 base barcode)-rGrGrG-3′; Eurofins MWG Operon) and libraries were amplified using Advantage 2 PCR system (Clonetech) with 16 cycles of a two-step program.
Based on a modified template of the service user questionnaire Section 1 of the survey targeting professional included the same list of user involvement activities.
Y-Family polymerases have unique structural features that allow them to bind damaged DNA and use a modified template base to direct nucleotide incorporation.
Similar(54)
Free-standing Ni-Co alloy nanowire arrays are synthesized via a modified template-assisted electrodeposition method.
The bismuth nanowires were first synthesized and purified using a modified template-based vapor deposition technique, resulting in nanowire diameters of either 20 or 250 nm, depending on the type of template employed.
The particles were prepared through a modified templated sol-gel self-assembly process.
In this work, we report on the fabrication of graphitized HCSs and yolk shell Au@HCS nanostructures using a modified templating method, in which smooth, uniform graphene layers were grown on SiO2 spheres or Au@SiO2 nanoparticles via metal-catalyst-free chemical vapor deposition (CVD) of methane.
A fraction of 1 2 μg of total RNA was used for cDNA synthesis with the MINT cDNA synthesis kit (Evrogen, Moscow, Russia), a strategy based on the SMART double-stranded cDNA synthesis methodology using a modified template-switching approach that allows the introduction of known adapter sequences to both ends of the first-strand cDNA.
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