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HIV MAbs 924 and 7B2 were conjugated separately to PAC and RAC by using a modification of the protocol described elsewhere23, 24.
In order to confirm internalization of ITs to the cells, we used a modification of the protocol described elsewhere45,46,47.
The QFS method is a modification of the protocol that was first described by McDonald and Webb [36].
In this study, it was hypothesized that a modification of the protocol to intermittent pacing would permit time for partial recovery of myocardial energetics leading to improved cardiac function and development of hypertrophy.
Once a desired level (>80%) of free spores were observed via phase contrast microscopy, spores were purified using a modification of the protocol of Zhao et al. (Zhao et al. 2008).
The assay was carried using a modification of the protocol described by Maguire et al. [10].
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To induce diabetes, rats were fed a high-fat diet (Special Diet Services) for three weeks, with an Atwater Fuel Energy of 5.3 kcal/g, comprising 60% calories from fat, 35% from protein and 5% from carbohydrate, according to a modification of the protocols of Reed et al. and Srinivasan et al.[ 18, 19].
Specifically, the current protocol is a modification of the Targeted Genotyping protocol commercialized by Affymetrix [ 54].
Our laboratory uses a modification of the CBD protocol when isolating rodent islets (see Appendix A for a annotated murine protocol and Appendix B for an abbreviated version).
The dIVT protocol, which requires only 50 250 ng of total RNA, is a modification of the IVT protocol where unlabeled cRNA is first synthesized followed by a second round of reverse transcription to generate cDNA and in vitro transcription to synthesize biotin-labeled cRNA.
Additionally, patients were treated using an insulin protocol, targeting a level of 100 140 mg/dL, which we developed on the basis of a modification of the Yale protocol [ 21].
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