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In these foci, an increase in the density of the actin network and a modification of cell shape were often noticeable (Fig. 4F and Fig. S4), suggesting alterations in the cytoskeleton.
Using toxicogenomics, we found a modification of cell metabolism, especially an alteration of mitochondrial function by underexpression of essential enzymes of the cellular respiratory chain, caused by these altered nanoparticles.
Together, these analyses confirm that the mutations lead to a modification of cell wall compositions in different organs.> -wrap-foot> Only significant differences between wild type and mutants are shown.
It is also possible that the effect of DEX results from a modification of cell adhesion molecules or other connexin-interacting proteins as has been suggested for other effectors [ 27, 28].
Since the percentage of proliferating cells we expect in the time frame of 24 and 48 hours is not high, we can hypothesize that this altered gravitational stimulus is not long enough to determine a modification of cell proliferation in this particular cell line.
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Trypanosome and MRC5 proliferation assay: Measurement of inhibition of the proliferation of MRC5 (human lung fibroblast) cells and T. brucei bloodstream-stage cells was performed using a modification of a cell viability assay previously described.
Yet during ovarian follicle cell development, at least four genomic intervals are over-replicated in a modification of the cell cycle, increasing the amount of DNA template available for transcription [ 55].
Decreased surface expression of co-stimulatory molecules and a modification of DC/T cell interaction have been reported [ 18- 20].
The mechanism responsible for inhibition of biofilm formation may involve a modification of the cell surface hydrophobicity or neutralization of adhesins involved in bacterial aggregation.
Hence, a modification of T-cell activation will be a valuable tool to disrupt the disease progression.
A modification of the cell-free protein synthesis protocol developed in our laboratory [ 16] was used to express TrEGI in an active, soluble form (Additional file 1: Figure S1).
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