If an entity is updated, the numeric version column is incremented by 1. Update statements always contain the version in the where-clause and if a concurrent update occurs, they do not match and the concurrent modification is detected.
Indeed, high levels of tyrosine nitration are found in Lewy body aggregates of α-synuclein and the same modification is detected in the soluble protein under conditions of cellular oxidative stress.
A significant effect modification was detected by physical activity (Wald test for interaction, P = 0.02), with effects of NO2 only in physically active people (HR 1.10 [95% CI 1.03 1.16]), and by smoking status (P = 0.02), with enhanced effect of NO2 in nonsmokers (1.12[1.05 1.20]) and none in previous (1.03 [0.98–1.09]) or current smokers (1.00 [0.88–1.00]).
Protein S-nitrosocysteine posttranslational modification was detected by a modified S-nitrosylation switch assay (S-Nitrosylation Western Blot Kit, Pierce, Rockford, IL, USA) as described previously [ 21].
A few years ago the H3T80ph modification was detected by mass spectrometric analysis, opening the possibility that, similarly to the cross-talk between H3S10ph and H3K9me3, H3T80ph could regulate the methylation of H3K79 by DOT1L.
However, an obvious parental bias of H3K36me3 modification was detected.
In comparison to HCT116 cells, H3K4me1 levels were much reduced at sites A through E in HEK293 cells although this modification was detected at the promoter region (Figure 3B).
Using i ∈ {1,…, K} to index each cluster, we evaluated the performance of the algorithms using a criterion that measures how well each ground truth modification was detected.
The double modification was detected by immunofluorescence (IF) using an antibody that specifically recognises these modifications when they are present in combination on the same histone H3 molecule.
Also, an H3F(7–50) peptide with one acetylation and one phosphorylation modification was detected by accurate mass, but no MS/MS spectra were acquired and therefore location of these modifications could not be determined.
This protein modification was detected by immunoblot and mass spectrometry (MS), and then comprehensively validated by chemical and biochemical methods.
