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At a modest depth of sequencing, viral transcripts can be detected at frequencies less than 1 in 1,000,000.
Thus, since budget is often a concern, it is recommended to increase the number of samples, which correspond to biological replicates, sequenced at a modest depth, rather than to increase sequencing depth in fewer samples.
We observed that all high confidence CDR3βs identified by RNA-seq (n = 9) fell within the top 2.1 % (n = 60) of CDR3βs detected by TCR-seq, ranked by abundance (data not shown), confirming that at a modest depth of sequencing, RNA-seq can identify the most abundant CDR3-encoding transcripts.
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Metagenomics alone, at a modest sequencing depth, cannot provide a highly resolved view of the community structure because of its bias toward sequences of the most abundant taxa.
Trump seems to believe that foreign affairs require only modest depths of thought.
Vladimir Putin and Recep Tayyip Erdoğan Trump seems to believe that foreign affairs require only modest depths of thought.
Since modest depths of shielding stop the vast majority of Solar Energetic Particles (SEP), the greater challenge is posed by the need to shield the crew from the Galactic Cosmic Rays (GCR), which include highly-charged and highly-energetic particles.
For example, the ENCODE RNA-seq standards [ 20] suggest that transcriptome profiling of polyadenylated positive samples require modest depths of sequencing, such as 30 million PE reads of length >30 bp, while for novel transcript discovery and strong quantification of known transcript isoforms deeper sequencing is required.
It was found that the cellular design was effective in significantly reducing the radial damage for an incoming projectile by up to 26%, with a modest increase in penetration depth of up to 12%.
This paper determines how well one can make population genetic inferences with shallow read depth using a modest number of individual genomes from the same species.
Fermented tomato passata adds a kick, if you care to make it, but good-quality dried and fresh porcini solve this conundrum, adding depth and transforming a modest soup into something luxurious.
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