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In each subject V3 amino acid residues revealed a mixture of sequences with low or high net charge predicting, as confirmed by two independent algorithms, CCR5 or CXCR4 coreceptor use respectively [39] [41].
Of the remaining multi-sequence macrofossils, four had a mixture of "correct" sequences and sequences of other species and one had a mixture of sequences from other species.
The sequence of Ogura-type unique region IV can be derived from a mixture of sequences in the reported Brassicaceae mitochondrial genome.
Each PCR product was a mixture of sequences from different genes, so many of the 11 81 clones obtained from one PCR reaction were independent.
All groups except the Ae. caudata-specific group 3 included a mixture of sequences of three to seven species of Aegilops/Triticum.
Our libraries contain a mixture of sequences from many members of the coral holobiont, including Symbiodinium, the cnidarian host and other associated microbes [ 8, 40].
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After a denaturation step (NaOH 0.5 M during 5 s) and a wash step for the removal of unlabeled complementary DNA of the amplicon, the biotinylated single-stranded DNA present on the streptavidin-coated Sepharose beads were transferred to a 96-well plate and used as template for the pyrosequencing assay with a mixture of sequencing primers.
The set of input reads contain a mixture of sequencing technologies including Illumina, 454 and Sanger.
The UGA10 clade contained a mixture of sequence types and was most similar to the IC11-clade.
These problems may be overcome by hybrid sequencing strategies using a mixture of sequencing methodologies, by new assemblers, and by sequencing more deeply.
One of the grand challenges in the study of metagenome data involves reconstructing the different microbial genomes from a mixture of sequence reads.
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