Uracil prototrophic transformants were serially passaged as described [ 26] to allow segregation of merodiploids containing a mixture of the integrated adhE expression cassette and the wild-type genomes.
A Chinese study examining oesophageal squamous cell carcinomas (SQCs) showed that only 9% out of 35 HPV-16-positive specimens exclusively harboured the episomal form, whereas the remaining 91% contained either only the integrated form (6%) or a mixture of episomal and integrated forms of viral molecules (86%).
Results: Our results showed that only 8.6% (3/35) of the HPV-16 positive specimens harbored exclusively the episomal form (i.e. E2/E6 ratio ≥ 1), whereas the remaining 91.4% contained either only the integrated form (5.7%, with E2/E6 ratio = 0) or a mixture of episomal and integrated forms of viral molecules (85.7%, with E2/E6 ratios > 0 but <1).
Using 12 cervical cancer samples positive for HPV 16 (unpublished data), we determined E2/E6 ratio and found that 92% of the samples had a mixture of episomal and integrated forms of HPV genome, and 8% of the samples had HPV integrated into the host genome.
Radio boards are a mixture of discrete and integrated analog and digital components.
When the E2/ E6 ratio was larger than zero and smaller than unity, this condition was considered as a mixture of episomal and integrated forms (Peitsaro et al, 2002).
The Greek health system is a mixture of (a) public integrated, (b) public contract and (c) public reimbursement models, comprising elements from both the public and private sectors and incorporating principles of different organizational patterns.
They provide an overall measure of exposure, absorption, and metabolic activation of a mixture of DNA adduct forming compounds, integrated with repair of DNA damage of an individual (Farmer 1994; Phillips and Arlt 2007).
Many hospitalized older patients suffer from a mixture of problems and therefore are expected to benefit from integrated care delivery.
As healthy functioning is a mixture of social, mental and physical status, sickness absence may be used as an integrated measure of healthy functioning and coping in working populations [ 1, 2].
