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In recent years, the mammary gland epithelium has been shown to be a mixture of differentiated cell populations in a hierarchical relationship with their stem and progenitor cells.
In Tsai et al.[ 59], 47 genes were found to be a specific MSC core gene signature when contrasting microarray data from four human perinatal tissues against a mixture of differentiated cells.
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However, EBs generally contain a mixture of cells, differentiated into multiple lineages, highlighting the challenge of achieving a homogenous cell population [36].
Dependent on the histological type, non-seminomas are composed of a disorganized mixture of differentiated somatic cell types and extraembryonic cells, together with EC cells.
Heterozygous CreA12 cells initially formed a mixture of undifferentiated and differentiated cells, but by day 9 had mostly differentiated with few remaining ES cells.
We previously showed that primary dissociated QNR cultures are composed of a mixture of progenitor and differentiated cells.
Thus, NSCs isolated from developing or adult brain grow as a mixture of undifferentiated and differentiated cells due the predominant asymmetrical mode of cell division [35], [36], [37], [38], [39], [40], [41].
Stem cells grown in minimal media that had been supplemented with conditioned media from fibroblast feeder cells produced a mixture of undifferentiated and differentiated colonies in response to treatment with Anti-MUC1* (Fig. 8C,D) or bFGF (Fig. 8E,F).
Stem cells that were cultured in conditioned media from fibroblasts plus bFGF generated a mixture of undifferentiated and differentiated colonies and the colonies were smaller than those produced by MUC1* stimulation.
Since tumors are considered a mixture of CSCs and differentiated cells, we analyzed the behavior of mixed populations of both cell types.
In absence of signals and intercellular communication, development would terminate in a salt-and-pepper mixture of differentiated cell types without any pattern.
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