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The hypercrosslinked monoliths in capillary columns were tested in an isocratic reversed phase liquid chromatography separation using a mixture comprised of acetone and six alkylbenzenes.
First, a mixture comprised of HRP (6 mg in 500 μL of D.I. water) and RITC (3 mg in 350 μL of DMSO) was stirred under dark conditions for 24 h at 4 °C.
Air pollution is a mixture comprised of several components, including ambient particulate matter (PM), gases, organic compounds, and metals.
Air pollution is a mixture comprised of several components, of which ultrafine particulate matter (UFPM; <100 nm) is of much concern, as these particles can enter the circulation and distribute to most organs, including the brain.
In a typical synthesis, bifunctional DNA Au nanoparticle conjugates were prepared by adding a mixture comprised of an equimolar ratio of the two oligonucleotides, e.g., 1 and 2, to an aqueous nanoparticle solution [ 14, 20].
However, since cinnamon bark has been typically used as in the form of a water extract, where the volatile ingredients are seldom found, it is likely that the established pharmacological activities of cinnamon bark depend on a mixture comprised of a variety of water-soluble components, thereby ensuring its safety as a traditional remedy.
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The nested GDCW/PLAS assay consisted of a 10-μL reaction with a master mixture comprised of 0.3 μM of each primer, 2 mM MgCl2, 0.125 mM dNTP, 1× buffer, 5% dimethyl sulfoxide, 1 unit of Biolase Taq, and 1 μL of DNA.
Seven compositions were prepared according to a simplex-centroid mixture design for a triaxial mixture, comprised of kaolinite, quartz and albite.
The triplex-associated fluorescent emissions from a 1 bp deletion triplex in a reaction mixture comprised of 1 ng hgDNA and mutant probe 3659delC-MUT25C (probe 8) remained constant throughout the 60 min incubation period and were 81% lower than those emitted from the reaction mixture containing the perfectly matched triplexes (Figure 3B).
The PCR reactions were carried out in a final volume of 20 μl of a reaction mixture comprised of 10 μl of Light Cycler 480 Probes Master (Roche), 0.2 μl of universal probe (Roche), and 4 μM of the primers.
The PCR reactions were carried out in a final volume of 20 μl of a reaction mixture comprised of 10 μl of LightCycler 480 Probes Master (Roche), 0.2 μl of universal probe (Roche), and 4 μM of the primers, according to the manufacturer's instructions.
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