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After washing with 10 mM potassium phosphate buffer pH 7.0 (buffer A), cells were suspended in 500 μL of buffer A, mixed with 500 μL of cyclohexanedione reagent [ ammonium acetate (10 g), acetic acid (10 mL), and 1,3-cyclohexanedione (0.25 g) in 100 mL of mili-Q water)], and incubated at 60°C for 1 h.
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The first injection was RCA-A (100 μg per injection) mixed with complete Freud's adjuvant (Sigma) and then RCA-A mixed with incomplete Freud adjuvant for the remaining injections.
Afterwards, the purified biotinylated puf-A were mixed with 10ug mRNA mixtures from embryos and ovaries.
"Had a blast playing a little mixed with @ryansweething tonight.
I have a mixed history with Erdrich.
It's a mixed bag with Mel".
It is a mixed neighborhood, with a large Christian population.
Now we finally have a mixed Parliament with different parties".
Authors seemed to have a mixed relationship with Kirkus.
US courts have a mixed record with respect to drones.
I would say a mixed city with mixed heights is a more interesting city.
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CEO of Professional Science Editing for Scientists @ prosciediting.com