The phrase "a mix consisting of" is correct and usable in written English.
It can be used when describing a combination of different elements or components that make up a mixture.
Example: "The cocktail is a mix consisting of vodka, cranberry juice, and lime."
Alternatives: "a blend of" or "a combination of".
The PCR was carried out in 25 µl of a mix consisting of 1× buffer, 200 µM dNTPs, 400 nM primers, 1 mM MgSO4, and 1 unit of KOD plus DNA polymerase (Toyobo, Osaka, Japan).
PCR products (5 20 µL) were added to a mix consisting of 3 µL Streptavidin Sepharose HP™ (Amersham Biosciences, Freiburg, Germany) and 37 µL binding buffer (Qiagen, Hilden, Germany) and mixed at 1200 rpm for 5 minutes at room temperature.
The reaction was stopped after 90 min incubation at 37°C with constant agitation by the addition of 50 µl of a mix consisting of metabolic poisons (400 mM citric acid, 10 µM rotenone, 10 µM antimycin and 3.5 mg KCN, pH 4.9).
PCR products (5-20 μL) were added to a mix consisting of 3 μL Streptavidin Sepharose HP™ (Amersham Biosciences, Freiburg, Germany) and 37 μL binding buffer (Qiagen, Hilden, Germany) and mixed at 1200 rpm for 5 minutes at room temperature.
A mix consisting of 4 μl of 5x buffer, 1 μl of RNase Inhibitor (Invitrogen), 1 μl of 5 mM dNTP and 1 μl of MMLV reverse transcriptase enzyme (200U/μl, Invitrogen) was added in a total volume of 20 μl, followed by 1 h of incubation at 45 °C.
mRNA and aRNA were reverse transcribed by adding a mix consisting of 8 μl 5 × First Strand Buffer (GIBCO), 2 μl Primer-Mix (oligo dT) and randomeres for mRNA or randomeres only for aRNA) (MEMOREC), 2 μl low C dNTPs (10 mM dATP, 10 mM dGTP, 10 mM dTTP; 4 mM dCTP), 2 μl FluoroLink™ Cy3/5-dCTP (amersham pharmacia biotech), 4 μl 0,1 M DTT, and 1 μl RNasin (20 40 U) (Promega).
To determine primer efficiencies and optimal quantification thresholds, a dilution series of a cDNA mix consisting of 4 ul solution from every sample was created.
For each of the 48 primer set lanes on the chip, a 'primer mix' consisting of 2.3 μL of a specific 20 μM forward/reverse primer (see Additional file 1), 2.5 μL of 2X Assay Loading Reagent (Fluidigm), and 0.25 μL low TE-buffer was prepared.
For the first kit, PCRs were performed in a reaction volume of 15 μL and a reaction mix consisting of 7.5 μL of AmpliTaq Gold® 360 Master Mix, 5.4 μL of water, and 0.3 μL (10 μM) of each primer.
The cycle sequencing reactions were performed in a reaction volume of 10 μL and a reaction mix consisting of 1.5 μL of 5X Sequencing buffer, 0.5-0.7 μL of BigDye, 1.2 μL (10 μM) of each primer and 1.0 μL of PCR product.
Most ice cream manufacturers make a standard mix consisting of milk, cream, sugars, and stabilizers, to which flavours may be added just prior to freezing.
