Sentence examples for a mitotic marker from inspiring English sources
To further confirm this, FACS analysis was performed using phospho-Histone H3 as a mitotic marker and α -actinin as a cardiomyocyte marker.
Progression through the S and M phases of the cell cycle, assessed by the incorporation of 5-bromo-2-deoxyuridine (BrdU) and the immunostaining of a mitotic marker, phosphorylated histone H3 (pH 3), respectively, was decreased in both cases in the zLMNA-MO1 morphants (Fig. 5A, f, j, see right graph).
Increased cardiomyocyte DNA synthesis evident by Ki-67+ cells in both the border zone and remote area suggested that some differentiated cardiomyocytes re-enter the cell cycle, which we further confirmed by co-localizing a mitotic marker (anti-pH3) with a myocyte protein.
To study the effect of MMS on mitosis, I first confirmed that mitosis in Hydractinia could be detected using a combination of a DNA stain and antibodies against Xenopus β-tubulin and human phospho-Ser10 Histone H3 (pH), a mitotic marker (Supplemental Figure S2).
The discs were also stained with an antibody to phosphorylated Histone H3, a mitotic marker.
In addition, to assess whether embryonic cells in the cultured Pold1−/− blastocysts entered the mitotic phase, we simultaneously immunostained them with an antibody against phosphohistone H3 (Ser-10), a common mitotic marker used to mark M-phase cells [21].
Intriguingly, the H2A H4 interaction was highly dependent on cell cycle stage, peaking during M phase in synchrony with aurora B-dependent phosphorylation of H3S10, a classical mitotic marker.
However, unlike Ser10, Ser28 is not phosphorylated until the onset of mitosis[68] and therefore serves as a more faithful mitotic marker.
However, these cells fail to express markers of cell-cycle progression, including the S G2 M markers, geminin, Aurora A and Plk1, and the mitotic marker, H3S10ph, indicating that these cells reside in a G1-prolonged or -arrested state (Williams and Stoeber, 2007).
BrdU incorporation (Figure 2M P) and phosphorylation of histone H3 at serine 10, which is known as a G2 phase and mitotic marker, were detected in the both pronuclei (PNs) of one cell embryos untreated or treated with 30 µM PJ-34 or 20 µM 5-AIQ 10 hrs after IVF (Figure 2E L).
