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Get a small vacuum.
Although in this experiment, starting with less than 1 μg of total RNA did not produce enough biotinylated cRNA for hybridization under the normal protocol, a minor change (using vacuum evaporation to concentrate samples before cRNA synthesis, and mixing the minimum 200 μl hybridization volume) should allow use of 1 μg samples with the standard protocol.
The lack of these bands within the concentrated ICF samples indicates that these samples did not contain major intracellular contaminations and that cell lysis played only a minor role during the vacuum infiltration process.
The vacuum pressure transmitted into the soil with a minor loss through the PVD length.
If the waveform period is longer than 5 s, a water domain has a minor effect on seismic waveforms and may be replaced by a vacuum.
"I'm a minor".
First, a minor quibble.
This was a minor annoyance.
In all, a minor triumph.
It is a minor miracle.
was celebrating a minor victory.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com