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A minimum spanning haplotype network also supported separation into three lineages.
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Minimum spanning haplotype networks were created with HapStar 0.5 [ 41], based on distance matrices produced with Arlequin 3.5 [ 42].
The minimum spanning haplotype network of genealogical relationships among the haplotypes revealed that haplotype Da25 is closest to haplotype Da24 in the Slovenian group of haplotypes in the Sava River drainage area from which it differed by a single mutation, while it differed by nine or more mutations from other Slovenian haplotypes from the same drainage area (Da22 and Da23).
A minimum spanning network of all haplotypes with complete sequences (1050 bp) revealed seven main clusters of haplotypes that corresponded to some of the subspecific classifications of cutthroat trout.
A minimum spanning network of mtDNA haplotypes was constructed using statistical parsimony [26] as implemented in the program tcs version 1.21 [27].
To illustrate the relationship between unique cutthroat trout haplotypes, we constructed a minimum spanning network using representatives of each unique ND2 haplotype and the program Arlequin v3.5 [ 29].
To characterize the frequencies and relationships of different haplotypes, a minimum spanning tree was constructed.
A minimum spanning network of all R. ridibunda haplotypes from Azerbaijan is presented in Figure 2. Regional and population diversity estimates are presented in Table 1.
A median joining algorithm was used to build a minimum spanning network to examine the evolutionary relationships among haplotypes, as implemented in Network 4.5 (http://fluxus-engineering.com/).
Relationships between haplotypes were also visualized as a minimum spanning network, using the Median-Joining (MJ) network algorithm implemented in the program network 4.1.1.0 [ 25].
COI sequence data were obtained for 156 individuals and yielded 83 unique haplotypes with no correlation to current taxonomic designations via a minimum spanning network.
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