Exact(11)
We required a minimum of two unique peptides identified using tandem mass spectrometry in conjunction with the SEQUEST algorithm [15] to confirm the presence of a protein in each organism.
Proteins were only included with a minimum of two unique peptides matched.
Protein identifications were based on a minimum of two unique peptides.
Most proteomic studies only report identifications with a minimum of two unique peptides or include the MS/MS spectra for single-peptide identifications.
In these studies, the filtering was set to a minimum R2 value >0.70 with a minimum of two unique peptides with four observations.
Conservatively, a gene product was confirmed expressed at the protein level only when a minimum of two unique peptides mapped to the particular gene.
Similar(49)
A minimum of three unique mutations was chosen to ensure that potential base mis-incorporation artifacts were excluded.
Minimum peptide and protein probabilities were set at 80 and 99%, respectively, with a minimum of five unique peptide matches per protein.
A Perl script was written to identify consecutive windows containing a minimum of ten unique piRNAs that held a single constraint.
A protein identification was accepted if a minimum of five unique peptides matched to the protein, which corresponded to a ≥99% probability of a correct identification (see Table 1).
In total, 32 proteins specifically enriched with UEAI (Table 1) were identified on the basis of both a minimum of five unique matching peptides, which corresponds to a ≥99.9% probability of correct protein identification, and their absence from the wild-type control and FUT1−/− UEAI lanes (lanes 1 and 4).
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