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As a minimum of three populations are required to test for IBD, we used only haplotypes of the subtropical mudprawn lineage.
As tests for IBD are only meaningful when performed on single regional genetic lineages [ 14] and require a minimum of three populations (see Methods), only samples from the subtropical lineage were included.
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Results from this study support substructure in Mauritian macaques and suggest a minimum of two populations and maybe three on Mauritius, with moderate admixture.
Since its collection, this stock has been kept in a minimum of four population cages (37 × 27 × 21 cm; L × W × H) at a large census size (> 5000 flies) under constant conditions (50% relative humidity, 12L:12D photoperiod, 25°C) with overlapping generations.
TNBC cell lines SCP6 were cultured in DMEM containing heavy C-labeled lysine supplemented with dialyzed FBS (Pierce, Rockford, IL, USA) while 4175 cells were cultured in DMEM containing light (C) lysine for a minimum of six population doublings as described in reference [ 24].
We required all SNPs to have a genotype call passing all stringent quality filters in a minimum of eight individuals per population (population-based filtering).
Worms were raised at 20° for a minimum of three generations or until the population was at least 250,000 individuals.
The d i statistic was calculated for autosomal SNPs in 300-kb windows, with a minimum of three SNPs per window, and defining the populations by breed.
This was confirmed by genotyping a minimum of three queens and 30 workers from each population at 33 microsatellite loci, as described in Fournier et al. (2005b) and Almany et al. (2009).
For each treatment condition from each line, six replicates were collected from a minimum of two independently thawed populations from each line.
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