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The incorporation of the target PSNR transforms the optimization to a constrained procedure to ensure a minimum of image quality that must be acquired.
The scan duration is only several seconds, which allows for a minimum of image quality degradation by breathing-induced and swallowing-induced motion artefacts.
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If we consider a pixel resolution of say 0.5 mm along the web and an image frame of 500 pixels in the same direction, then for a speed of 20 m/s, we have to process a minimum of 80 image frames per second (12.5 ms/image) to achieve real-time operation.
This analysis requires an image stack of a minimum of 10 20 images, depending on intensity, to provide the values of the average and variance at each pixel used to calculate N and B. These parameters are related to the true number of particles and true brightness of the fluctuating particles according to the relationships presented in this article.
For each cell line and treatment, a minimum of three images were taken and within each image a minimum of six measurements of distinct mitochondrial regions were taken to give n=18.
A minimum of 20 images with a mean of 4 cells per image were taken per condition, per experiment.
A minimum of three images for each sample was captured, and representative images were included in Figure 1.
We obtained a minimum of three images.
A minimum of 10 images from each sample was analyzed to assure reproducible results.
On one hand, it is shown that, using Bootstrap simulations, the standard deviation of the classification results can be very important if too small training sets are used; moreover a minimum of 5000 images is needed in order to perform reliable steganalysis.
Except where indicated, a minimum of 100 images were acquired and employed for quantitative analyses.
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CEO of Professional Science Editing for Scientists @ prosciediting.com