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For each patient, 2 or more biopsies were taken from the second/third portion of the duodenum (at least a minimum number of 4 samples), and at least 1 or more biopsies were taken from the duodenal bulb [ 2, 12].
Therefore, in order to distinguish Iγ1 from the remaining subclasses, PCR was carried out with primers that contained a minimum number of 4 naturally occurring base alterations and 3-4 additional changes that were intentionally introduced into the primer sequence.
The search is accomplished with the ProSight search engine and requires a minimum number of 4 matched fragments, with only the top hit (the most confident P-Score) returned from the search.
Simple sequence repeats penta-, tetrand and tri-nucleotides long, with a minimum number of 4, 5, and 6 tandem arrays of the core repeat, respectively, were retrieved from the grape sequence available at, using a modified version [ 9] of the software Sputnik, developed by Chris Abajian at the University of Washington.
Archaeolemur edwardsi sample CH126 instead yielded a 190-bp cytochrome b sequence (Table 1) that exhibited only a minimum number of 4 substitutions with Archaeolemur majori haplotypes while the 94-bp cytochrome b sequence obtained from Hadropithecus stenognathus sample CH421 was found to exhibit a larger divergence from the Archaeolemur sequences (24 substitutions).
As a conclusion from these results, four minimum requirements were formulated for the ELISPOT protocol: (1) perform triplicates for each test antigen (2) do not use allo-APC (3) add a resting time to increase the proportion of living cells seeded and (4) use a minimum number of 4 × 10 PBMC per well.
Similar(54)
An optimization problem arising in the design of optical networks is shown here to be abstracted by the following model of covering the edges of a bipartite graph with a minimum number of 4-cycles, or K2,2: Given a bipartite graph G="(L,R,E) over the node set L∪R with E⊆{[u,v]:u∈L,v∈R}, and the implicit collection of all four-node cycles in the complete bipartite graph over L∪R.
Only localities with a minimum number of 9 individuals sequenced available in the literature16,17,18 were chosen for this second analysis.
For the inherent parameter variability that was chosen as default setting a minimum number of 50 branching points should be observed.
We choose to use the default grouping values: the source spectrum will be grouped to a minimum number of 15 counts per new channel and the background spectrum will be ungrouped.
A numerical approach based on finite volume method was used and, for each value of defectiveness considered, ranging from 0.25 to 10%, a minimum number of 200 different geometries were analyzed, in order to obtain statistically meaningful results.
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