When the transient detector signals a transient to the envelope estimator, segments of shorter duration in time are defined by the envelope estimator, starting with a minimal segment, the leading border of which is placed at the onset of the transient.
In order to disrupt possible interactions between DDX3X and its viral or host factors responsible for DDX3X stimulation of HCV propagation, we first mapped the minimal segment of HCV core protein that is capable of interacting with DDX3X.
Some of the chromosome anomalies, including gains in three independent minimal segments of RNO20 (RNO20p11. 20p12 and 20q12) and in a small segment of RNO 12q11, appeared to be more specific to this tumor model, since they have not been reported in other mammary tumor models [ 33].
We first used in cellula imaging to define the minimal segments of MBP required for interaction with Fyn SH3 as demonstrated by phenotype, followed by solution NMR spectroscopy of a recombinant construct of this region and the Fyn SH3 domain.
Segments of uniform copy number value for each patient were compared to compute the set of unified breakpoints across the whole dataset, and the so determined set of minimal segments of change were used as features for the clustering procedure.
In constructing this peptide, we first used in cellula imaging to define the minimal segments of MBP required for interaction with Fyn SH3 as assessed by morphological phenotype of the immortalized N19-cell line, representing an early-developmental oligodendrocyte [ 10, 44, 45].
The refinement procedure produces a minimal subdivision of segments from all pairwise comparisons ensuring the segments are non-overlapping.
The advantage of this method is that the NZ is derived from the load-deflection curves without involving any arbitrary choices, but the deformation at zero load does not per se mark the borders of minimal segment stiffness.
One node A is the child of the other node B if and only if the segment corresponding to B is the minimal segment containing the segment corresponding to A. In case that one node has more than two child nodes, we can always add some intermediate nodes so that any node has at most two child nodes.
Clearly for the healthy patients this results in a minimal number of useful segments such that there were fewer than 10, in this case the deficiencies in each individual were supplemented by random extra segments from other patient groups.
