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Using a plate-based, fluorescence polarization (FP) assay, we identified a minimal region of LZ4 that suppresses binding of HSF1 to the HSE.
A minimal region of homology of 30 to 50 bases flanking the fragment to be inserted is all that is required for targeted mutagenesis.
A minimal region of 8p21-22 was found to be frequently deleted in HCC.
A useful method for pinpointing a minimal region of interest and potentially gene(s) is the occurrence of recurrent chromosome changes, and particularly recurrent chromosome breakpoints.
This has now been narrowed down to a minimal region of about 500 kb that contains thirteen annotated genes, only three of which are expressed in hPSCs: HM13, ID1 and BCL2L1 (Amps et al., 2011).
A minimal region, capable of activating Dpy2 splicing in vitro, was mapped to the first 90 nucleotides of exon 23 (Figure 1, D90).
Similar(31)
Here we confirm the importance of the rod positively charged C-terminus region for MHC-IIB self-assembly and paracrystal formation in vitro and for cytoskeletal association in vivo In the negatively charged region no particular sequences were identified as important for self-assembly, yet a minimal area from this region is necessary.
In contrast, no particular sequences in the rod region with net negative charge were identified as important for self-assembly, yet a minimal area from this region is necessary.
Therefore, alignment of our LOH data with the physical map of the 11p telomeric region delimited a minimal area of loss common to all of these tumors between markers D11S1997 and D11S4905.
For example, on chromosome 20, the high-amplitude approach identified a small minimal region that was not evident from the low-amplitude analysis.
Moreover, fine mapping of these deletions implicates a 170 kb minimal region that includes p16 and excludes p15.
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