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Exact(4)
The double staining of CD68+ cells cultured for 3 weeks showed the presence of vimentin, ASMA and a minimal expression of smoothelin and desmin.
A 1.5 fc relative to the control and a minimal expression of 200 fluorescence intensity was used as cut-off.
DCs showed a high expression of DC-SIGN, CD1a and CD11c, low expression of HLA-DR, CD80 and CD83 and a minimal expression of CD14 at flow cytometric analysis.
A 1.5-fold change (fc) relative to the control and a minimal expression of 200 fluorescence intensity was used as cut off, and we detected 388 genes with increased and 381 genes with decreased expression.
Similar(56)
Intracellular inhibitors are also affecting sensitivity to anti-CD95 but a minimal expression level of CD95 is required, since the addition of cycloheximide alone to inhibit the synthesis of short-living antiapoptotic proteins can sensitise SW948 to anti-CD95, but cycloheximide as well as upregulation of CD95 membrane expression by interferon- γ were needed to sensitise Colo320 to anti-CD95.
Those genes which displayed a minimal expression level of -2 and an up- or downregulation of at least 2-fold were selected for pathway analysis, using DAVID and KEGG provided by the National Center for Biotechnology Information (NCBI).
A minimal expression change of 3.0 and 0.3 fold had to be reached to be considered as an up-regulated or down-regulated candidate gene respectively.
Highly expressed genes were selected with at least a ten fold over-expression when compared to normal neural tissue and a minimal expression level of at least 5 or 10 counts per 100,000 tags (in respectively grade III and IV tumors) in 30% of the corresponding tumor type.
In this study, minicircle, which is a minimal expression vector free of bacterial sequences, was employed for MSC transfection as a non-viral gene delivery method.
A minimal expression fold change of ≥2 in all three replicates is indicated with a +.
All 600 genes show a minimal expression fold-change of two and a p-value equal to or below 0.05.
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