Exact(19)
In this study, minicircle, which is a minimal expression vector free of bacterial sequences, was employed for MSC transfection as a non-viral gene delivery method.
The double staining of CD68+ cells cultured for 3 weeks showed the presence of vimentin, ASMA and a minimal expression of smoothelin and desmin.
A minimal expression change of 3.0 and 0.3 fold had to be reached to be considered as an up-regulated or down-regulated candidate gene respectively.
A minimal expression fold change of ≥2 in all three replicates is indicated with a +.
A minimal expression by the endothelial cells of gonadal blood vessels has also been reported [ 3, 4].
All 600 genes show a minimal expression fold-change of two and a p-value equal to or below 0.05.
Similar(41)
Although hMSC have a common minimal expression marker profile of surface receptors, additional distinct surface molecules and metabolic alterations can be detected between different hMSC populations which may determine their functional diversity within the different tissues.
A score of 0 represented minimal expression, whereas a score of 3 represented abundant expression of a marker.
A score of 0 represented minimal expression, while a score of 4 represented abundant expression of a marker.
Acceptable inducibility of the gene's expression requires a high level of its expression in the presence of Dox and a minimal basal expression in the absence of Dox.
The condition with the maximal expression gained a rank of "1", and the condition with the minimal expression gained a rank of "3".
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