Sentence examples for a minimal alignment from inspiring English sources

First, a blastx search [221] in the NCBI nr.aa database was performed, and all sequences producing an E-value<1E-3 and a minimal alignment length of 33 residues were considered a hit.

A minimum score of 250, corresponding to a minimal alignment length of 50 bp, was applied following the usual recommendations to map orthologous ESTs [ 29].

Pig centered, multiple alignments were formed from the pairwise alignments with the roast program from the tba/multiz [ 79] package using a minimal alignment block size of 40.

The Shannon diversity index was computed using mothur [ 60] on 16S rRNA sequences extracted by BLASTN against the NCBI 16S microbial database using E-value cutoff 1e-5 and a minimal alignment length of 50 bp.

We then applied a stringent criterion to preserve homology relationships between sequences [ 37], imposing a minimal alignment coverage of > = 80%% between any pair of sequences with > 30%% identity, eventually retaining 131,162 environmental sequences.

At a 90% identity threshold and ≥5% coverage of the complete bacterial genomes (for a typical bacterial genome of 4 Mb, it corresponds to a minimal alignment length of ∼200 kb, >100-fold longer than the 16S rRNA gene and thus provides more confidence) or ≥0.5% coverage for fungal genomes, the 48 most abundant bacterial and 2 fungal species were identified (SI Table S2).

If a protein was not complete but still exceeded a pre-computed minimal alignment score, then we called the protein 'partial'partial

BLAST (balstn) was used with the default options, with an exception that the minimal alignment length was set to 7 bp.

As a consequence, the specific adjustment of parameters such as E-value, word length, minimal alignment length and percent identity for each metagenomic dataset can complicate a BLAST-based comparative analysis.

Our algorithm employed 2 quality assurance criteria for reference sequences identified in BLASTn analysis: minimal alignment length of 98% and annotation as a validly described bacterial taxon (28 ).

The Schwann cells seeded on scaffolds of random nanofibers demonstrated a disorganized actin meshwork, in agreement with the observations of random organization and minimal alignment.