This catheter was connected to a Hamilton syringe powered by a mini-pump KDS310 (KD Scientific, Holliston,MA, USA).
In order to further support our hypothesis that ephrin-B3 deficiency is crucial for the aforementioned observations made in ephrin-B3−/− mice, knockout animals received continuous intraventricular infusion of soluble ephrin-B3-Fc via mini pumps for as long as 4 weeks.
NGF or saline was subcutaneously administered by an osmotic mini-pump for a period of 3 14 days.
After 13 days, at PND 107, all rats received a new mini pump under isoflurane anesthesia.
Isoproterenol (ISO) was given to C57BL mice with or without ARB (olmesartan) treatment and to AT1aR−/− mice by a subcutaneously implanted osmotic mini-pump for 11 days at a rate of 15 mg/kg/day.
The analytical procedure employed a multicommuted flow analysis manifold, using solenoid mini-pumps for fluid propulsion.
A subinotropic dose of ouabain (50 µg/kg/day) was constantly administrated by osmotic mini-pumps for the first 4 weeks.
In this study, we show that compared to mice receiving PBS, rhGas6 administration directly to the corpus callosum by osmotic mini-pump for 14 days enhanced the clearance of lipid-laden debris, remyelination and reduced axonal damage that resulted from 4 weeks of ingestion of cuprizone in powdered chow.
Mice were treated by osmotic mini-pump for 4 weeks with neutralizing Anti-ASP or control non-immune IgG (NI-IgG).
In addition, to verify that infused Ang-(1 7) was detectable in the circulation, a separate group of control rats received vehicle (0.9% saline, n = 5) or Ang-(1 7) (subcutaneous 24 μg·kg−1 of body weight·h−1, n = 10) via osmotic mini-pump for 10 days, and rats were killed and plasma Ang-(1 7) levels measured.
Ekdahl et al. (03) used LPS that they delivered into the cortex continuously by an osmotic mini pump.
