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In this study, we chose a mild procedure with a one-step sucrose gradient to purify the yeast LDs in order to avoid losing weakly-associated proteins (Vindigni et al., 2013).
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This is a sufficiently mild procedure that most FAs, including polyunsaturates, epoxides, and cyclopropenes, are unaltered [9].
In the present work, the room temperature dried algal biomass was only manually ground, which is a very mild procedure for disrupting the algae cell wall; therefore, the enzymatic hydrolysis results reported herein are quite promising.
A one-step, mild procedure based on coaxial electrospinning was developed for incorporation and controlled release of two model proteins, BSA and lysozyme, from biodegradable core-shell nanofibers with PCL as shell and protein-containing PEG as core.
A mild synthetic procedure was developed to prepare aldehyde modified HA by incorporating an amino-glycerol side chain via amidation reaction and selective oxidation of the pendent group.
By harnessing the MN's reversible swelling/deswelling property, insulin, a model protein drug, was loaded in the swellable tips using a mild drop/dry procedure.
Recently Atkins et al. [15] found that "c-disordered" H+-birnessite with a poorly crystalline, hexagonal layer symmetry can also be transformed into todorokite under a mild reflux procedure.
For NW synthesis, Ge 001) substrates are prepared by a mild sputtering/annealing procedure: Surface cleaning is performed by 4 cycles of Ar sputtering (830 V, 20 min) and annealing at 830°C by direct current heating.
(At the very least, this horrific prospect removes the comfortable assumption that lethal injection is basically a mild surgical procedure, the equivalent of having your appendix out).
The slices were subject to a mild digestion procedure (see Methods) that is expected to selectively extract GABA from the cytosolic compartment and leave the vesicular stores intact.
Induction of a putative negative affective state using a mild stress procedure resulted in rats making fewer reward responses to ambiguous 'probe' tones, indicating decreased anticipation of a positive outcome.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com