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We recently described a method for reconstituting peripheral nerve (PN) sheaths using adult Schwann cells (SCs).
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We first developed the GRecon method for reconstituting the 1.7 MDa mitochondrial supercomplex I1III2IV1 from digitonin into proteoliposomes in order to compare its structure in a membrane environment to the three-dimensional map of the amphipol-solubilized supercomplex we determined recently by single-particle electron cryo-microscopy (cryo-EM).
Basic methods for reconstituting membrane proteins into phospholipid vesicles were developed quite a number of years ago [ 1], facilitating fundamental discoveries in such diverse cellular processes as metabolism, solute transport, protein trafficking, and signal transduction.
All these conventional methods for reconstituting membrane proteins into liposomes are therefore time-consuming, labor-intensive, and unpredictable.
Techniques to preserve female eggs or fertilized embryos have not been developed for salmonids, so Thorgaard and Cloud (1993) and O'Reilly and Doyle (2007) reviewed two methods for reconstituting original wild populations from cryopreserved sperm.
Haloduracin biosynthesis was recently reconstituted in vitro(6), providing a method for the production of haloduracin mutants (29).
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In this chapter, we describe a protocol for reconstituting the concentrative nucleoside transporter from Vibrio cholerae into proteoliposomes.
GPC serves as a precursor for reconstituting a nerve cell membrane component.
Such a mechanism for reconstituting a functional UAA is known to occur in some mammalian mitochondrial transcripts [ 40].
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