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The IKK assay was performed by a method described previously (Takada et al., 2004).
-131I]p IV was prepared using a method described previously [23].
Haemolytic and lecithinase activies were determined using a method described previously (Matarante et al. 2004).
Ozonated samples were preconcentrated using solid-phase extraction (SPE) based on a method described previously [31].
The PLA-PCL-TPGS nanoparticles was further modified by thiolated chitosan using a method described previously [31].
Total cellular RNA was extracted from R. eutropha strains using a method described previously ([Brigham et al. 2010]).
The surface modification of the PLGA-TPGS nanoparticles was carried out by a method described previously [14].
13C-labeling of proteinogenic amino acids was analyzed using a method described previously with some modifications (You et al. 2012).
PG was extracted from the E. coli DH5α strain by following a method described previously (Desmarais et al., 2014).
The DPPH radical-scavenging activities of the compounds isolated from G. paraguayens were measured using a method described previously (Chen et al. [2008]).
They were prepared according to a method described previously [33], [34].
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