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A maximum of three cells per clump was accepted.
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(Note: all embryos were laser treated using an average laser power of 45 mW with a beam dwell time of 100 ms and a galvo pulse number of 3. A total of 3 transient pores per blastomere were created with a maximum of two cells laser manipulated).
Each lymph node sample gave a maximum of five cell fractions for RT-PCR, and from a total of 71 patients we successfully obtained both IMS and RT-PCR data.
Thus, with the improving capacity of the PacBio SMRT cells, we predict that a maximum of three SMRT cells should be sufficient to obtain the entire complement of replicons of an actinomycete genome of around 8 Mb in single contigs.
Within one enlarged portal field only, a maximum of three immunopositive (red) cells were found at day 11 after PH (Fig. 7h).
Using this method we were able to generate GFP-positive wild-type and myospheroid mutant (mys ) hemocytes in the dorsal patches area (Fig. 1C), with a maximum of three GFP-positive cells in the selected region.
Thus, lesions must be formed by a maximum of two inoculated cells.
Techniques such as fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) have shown that there is an average of one fetal NRBC per milliliter of maternal peripheral blood, with a maximum of six fetal cells per milliliter of maternal peripheral blood [ 32, 33].
A maximum of three live or dead cells can fit into each square, although only one living macrophage can be present at any one time (i.e. two living macrophages cannot be present in the same square; Figure 8).
BECs were subcultured a maximum of six passages and cells from each passage were frozen for later use.
The cells were subcultured before reaching confluence for a maximum of three passages.
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