The metastases in the stained lung sections were characterized by a higher tissue density compared to normal lung tissue (Figure 6), intense VEGFR2 staining (Figure 6A1,B1) and a marked PyMT-staining (Figure 6A2,B2).
Very few c-Myc positive cells could be detected in the thymus by immunofluorescent staining of wild type 6 week old litter mate mice however the remnants of thymic epithelium of K5CrexRac1flox/flox mice showed a marked increase (Supplementary Figure S2A D).
Postnatal Tpr-Met mice had significantly increased heart mass (0.296 g±0.080 vs control 0.160 g±0.056, p<0.005) and heart/body weight ratio (2.3 fold increase, p<0.005), indicating a marked ventricular hypertrophy (Figure 5C).
While only a few TUNEL-positive cells were observed in TLR ligand-treated wild-type mice, IECs of Casp8ΔIEC mice showed a marked TUNEL positivity (figures 3B and 4A).
Facial bones were generally intensively cut-marked (Figure 5A).
As shown in Figure 4, a marked increase in the number of TUNEL-positive cells was observed in the group treated with cisplatin in a dose-dependent manner, while the number of TUNEL-positive cells was significantly reduced in the group receiving candesartan in addition to cisplatin.
Of note, transfected cells showed an increase in cells residing in G0 and G1 phases accompanied by a reduction of cells in S phase (figure 3F), a marked increase in p21 expression (see online supplementary figure S3C), and most importantly increased chemoresistance to gemcitabine (figure 3G).
That's a marked improvement, considering the figure had been a third higher or more just a few years ago: 36% in 2009, 34%% in 2010, and 33% in 2011.
We observed a marked decrease in migration (Figure S4A).
Strikingly, cells expressing CXCR4CFP and CCR5YFP showed a marked enhancement of FRET (Figure 4B and 4D).
We used Western blot analysis to detect dual phosphorylated ERK1/2 (dpERK) and show that in THP-1 cells CCL2 induced a marked activation of ERK1/2 (Figure 3B, lane 2).
