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The phrase "a major negative influence" is correct and usable in written English.
You can use it when discussing the impact of something that is significantly harmful or detrimental.
Example: "The constant exposure to violent media can be a major negative influence on young people's behavior."
Alternatives: "a significant adverse effect" or "a substantial detrimental impact".
Exact(5)
To him, the integrity of the strip and its artist would be undermined by commercialization, which he saw as a major negative influence in the world of cartoon art.
However, proximity to the single integrally protected area in the landscape maintained an overriding positive influence on elephant abundance, and logging roads – even subject to anti-poaching controls - were exploited by elephant poachers and had a major negative influence on elephant distribution.
These constraints and the escalating paucity of resources have had a major negative influence on quality of education.
In addition, weight gain (or fear of weight gain) is a major negative influence on treatment adherence [ 15].
Though controversy surrounds the extent and means by which some of the mechanisms of the aging process affect quality of life, it is well-established that the general process does have a major negative influence on physical well-being.
Similar(55)
However, radiation damage remains a major negative consequence of radiotherapy.
Obesity is a major negative determinant of breast cancer outcome.
"Sun breaking $20 is a major negative, technically.
I tended to act quickly when I saw a player become a negative influence.
a positive influence, a negative influence.
"Sometimes that has a little bit of a negative influence.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com