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The theoretical calculation results indicate that the two asymmetric compounds behave a main transition from the HOMO − 1 to the LUMO or from the HOMO to the LUMO + 1 upon excited.
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The increase in peptide concentration leads to a gradual decrease in the main transition enthalpy to an almost abolishment at P/L ratio of 1/10.
Comparison of the heating and cooling runs shows that RT42, while having a strongly first-order main transition (large latent heat), does not exhibit strong supercooling of this 42(,^{circ })C transition.
GUVs from pure DPPC can only be prepared by electroformation at a temperature above the main transition temperature.
Contrarily to what was observed with DMPC, in the case of DMPG, DSC measurements showed a strong perturbation of both the pre-transition and main phase transition temperatures by penetratin which leads to a gradual decrease in the main transition enthalpy with the increase in P/L ratio to an almost abolishment at P/L ratio of 1/10.
The main transition is a large peak between 30 and 45 (^{circ })C.
The main transition shows a distinct shape in the cooling curves.
DSC indicates that the peptide decreases slightly the enthalpy of the main transition with a concomitant broadening (Figure 2F).
As observed by DSC, the peptide did not induce any significant changes in the DEPE main transition but a slight decrease in the temperature of the Lα-HII transition (from 64.2°C to 62.6°C) (Figure 4J).
The broadening of the main transition peak revealed a lesser DPPC melting cooperativity for Py-met-chol than for cholesterol.
In lieu of the steep pretransitional drop to a lower CSA value above 40 °C, Δ σ in the mixture exhibits a decay with indications of a two-step feature associated with the reduced main transition and the first new transition in DSC.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com