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We then went on to test the dissociation of p62 from mono-ubiquitin and linear tetra-ubiquitin using FRAP and found that p62 is much more tightly bound (i.e. has a lower off rate) to tetra-ubiquitin chains than to mono-ubiquitin.
The improved potency of SGC0946 in cellular assays was attributed to a lower off rate together with enhanced cell membrane permeability.
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Still, I can't shake the feeling that, with a few hardware (too late to add a headphone jack?) and software tweaks (and a lower, off-contract price point), Palm could help reignite that fire for some.
In fact, if the PB1 domain increases the avidity of p62 towards surface-localized LC3B via oligomerization, this would result in a lower off-rate of the wild-type protein compared to the non-oligomerizing delta PB1 PB1.
Therefore, one possible interpretation of these results is that the oligomeric wild-type p62 has a lower off-rate from LC3B clustered on a surface than the non-oligomerizing mutants.
Retrieving the stored data involves bouncing the laser beam (at a lower power) off the surface of the disk.
The implications of applying a "lower cut off" to peak suctions determined from wind tunnel tests is explored in the context of structural reliability.
A lower cut off point of 1000 steps, however, seemed too high and thus inappropriate for a chronically ill and mobility-limited population.
Only 15% were ART-naïve and a lower cut off for nephrotic range proteinuria Upr/cr ratio >1.0 was used [ 17].
The latter study and our results come to a lower cut-off than the recommended cut-off value of 100 ng ml−1 by the manufacturer (Eiken Chemical Co).
The 4DSQ anxiety scale employs two cut-off points, based on clinical experience [ 28], a lower cut-off point with a relatively high sensitivity and a higher cut-off point with a relatively high specificity.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com