Exact(2)
The operational case definition of CCD, used in this study, may have a low level of specificity and, thus, runs the risk of misclassifying individual colonies, which in turn can bias results [10].
However, equally high values are obtained if tissues of different origin like liver and tumor are compared (253 (L/T) + 75 (T/L) = 328, Table 2c) indicating a low level of specificity and accordingly many false positive genes that are actually not suitable as potential target genes.
Similar(6)
However the majority of the existing PWMs provide a low level of both sensitivity and specificity [ 55].
Both classifiers performed inferiorly to the 42 gene-transcript classifier, with the GWAS DEGs classifier achieving an accuracy rate of 59.0 ± 4.6% with sensitivity 55.0 ± 5.2% and specificity 72.0 ± 9.0% and the only DEG's classifier demonstrating an accuracy of 76.0 ± 3.9% with sensitivity of 83.0 ± 3.9% but low level of specificity 53.0 ± 10.0%.
Then, two rounds of nested PCR were performed with nested C gene primers to enhance PCR production specificity, ultimately yielding the target sequences with a low level of background amplification.
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