Exact(4)
This result suggests that there were some reads that Bowtie2 could not align or gave a low alignment score when the combined reference strategy was used, which were likely reads with highly similar sequences in the human and mouse genomes.
Clade 4 comprises six proteins belonging to S. cerevisiae and V. carteri, with a low alignment identity (42%).
As expected, unwounded skin displayed abundant collagen with a basketweave appearance (largely randomly oriented fibers) with a low alignment (0.57 ± 0.003; Figures 4A 4C).
Similarly, removing 9 bp from the 3′ end resulted in a low alignment percent (73.27%), although this was higher than untrimmed read mapping probably as a result of the reduced mapping specificity of shortened reads.
Similar(56)
We show that even at a low SNR level of 0.5 and a typical range of tilt angles between -70° and +70° our method can still achieve a very low alignment error (Table 1).
Figure 4B shows two extreme examples, a deep matrix (VT120) correcting a high identity alignment (dash-dot line) and a shallow matrix (VT40) correcting a low identity alignment (rounded-dash line).
A primary sequence alignment of D. melanogaster AANATA with sheep serotonin N-acetyltransferase produced a low sequence alignment score (<12%), and the catalytic core of the mammalian ortholog is not conserved in the fly enzyme.
Reads failing to be unambiguously aligned to a unique location would be marked as having low alignment quality, which would in turn affect the ability to identify any genetic variants it contains.
This relatively low alignment rate is an intrinsic characteristic of SOLiD 4 System sequencing [ 20].
Surprisingly, the final library yields were very low and the results obtained from sequencing of these libraries were not satisfactory: high duplication rates coupled to low alignment percentages suggested an insufficient library complexity.
In this paper, we proposed to use ultraviolet-curable liquid crystal polymer (LCP) in conjunction with polyhedral oligomeric silsequioxane (POSS) nano-particles (2 5 nm) to develop a low temperature alignment technology with wide range of liquid crystal pre-tilt angles.
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