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Inactivation of rep in InvA or InvBE mutants did not cause any loss of plating efficiency, although InvBE rep overnight colonies on LB were quite small.
As a further test, S. coelicolor M1152 pBF20 pBF22 pBF24F24 was subjected to two rounds of sporulation without selection, and there was no loss of plating efficiency on any of the selective plates.
Besides these known changes in cartilage, in vivo μCT showed that papain injections induced a loss of medial subchondral plate thickness, formation of subchondral plate pores, and loss of trabecular bone.
Untreated pdgfra mutants have a loss of the ethmoid plate, which leads to a significant reduction in the length of the palate (Fig. 1C, asterisk; supplementary material Fig. S2) and have slightly smaller pharyngeal skeletal elements (Fig. 2C,G-I; supplementary material Table S1).
In angular stable plating, a loss of reduction often causes varus collapse, which leads to screw perforation.
SSS substitutions C1564S and W1570C were previously shown to induce a loss of attachment and spreading of fibroblasts plated onto fibrillin-1 fragments containing domain TB4 (cbEGF22-TB4-cbEGF23), indicating an impaired interaction of these fragments via the RGD motif with the integrin αvβ3 (15).
Spinal fusion by means of a screwed plate generates a loss of intervertebral mobility, whereas a spinal dynamic implant (SPD) allows relative displacements and thus preserves the adjacent segments and discs.
They found a hyperplasia of the growth plate and a loss of the classical cellular architecture of this specific cartilage.
In addition, depletion of Bsk by RNAi resulted in a loss of the p-JNK signal in the plate-based assay (data not shown), confirming that the antibody is specific for p-Bsk in samples of fixed cells.
Attention is drawn to the possible loss of plate-group compression during the formation process, and how this may be avoided.
Specifically, treatment with raloxifene for 48 h induced dramatic cell death evidenced by cell rounding, membrane blebbing, and loss of plate adhesion.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com