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A loopful of 18 h broth containing organism was transferred to a clean glass slide, Gram stained and examined under microscope.
Completed test was performed by plating a loopful of broth from a positive BGLB tube on to an Eosine Methylene Blue (EMB) Agar plate.
A loopful of the glycerol stock of each isolate was transferred onto nutrient agar, streaked and incubated at 37 °C for 18 24 h.
A loopful of each of five Escherichia coli strains isolated from wound was suspended in 10 ml of sterile distilled water.
A loopful of each of the isolated colonies from M7 agar plates containing heavy oil was transferred to 3 cm diameter wells, containing 5 ml of Luria broth.
Inoculum was developed by inoculating a loopful of pure culture into a medium consisting of 1 % keratin substrate and 0.2 % yeast extract (pH 7.50).
A loopful of the enrichment culture was streaked onto thiosulphate-citrate-bile salt-sucrose agar used for the selective isolation of Vibrio strains (TCBS agar, Scharlau Microbiology, Spain).
Briefly, first stage culture was initiated by inoculating 25 mL of sterile liquid medium with a loopful of freshly grown culture, under laminar airflow cabinet.
A loopful of colony from an agar plate was picked up and aseptically transferred into a 100-cm3 medinm in a 250-cm3 conical flask.
Confirmed test was carried out by transferring a loopful of broth from a positive tube into Brilliant green lactose bile (BGLB) broth, followed by incubation at 37 °C for 24 48 h.
Inoculum was prepared by inoculating a loopful of strain ZX01 growing on Gause's No. 1 agar plate for 72 h into a 250 mL flask containing 100 mL Gause's No. 1 liquid medium.
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CEO of Professional Science Editing for Scientists @ prosciediting.com