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The growth of Pseudomonas putida epI on ethoprophos appeared to be a logarithmic mode between 5 and 35 h of incubation [38].
Data were obtained from fluorescence channels in a logarithmic mode.
A total of 5,000 CD61-positive events were collected with all light scatter and fluorescence parameters in a logarithmic mode.
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The red cell population was lysed using UtiLyse™ (Dako Cytomation) and the samples were analyzed on a FacsCalibur flow cytometer (BD Biosciences) in the logarithmic mode.
Then the amplitude of oscillations starts to increase exponentially (in the logarithmic mode) until the next quench signal resets them.
Unbound platelets were measured with forward and side scatter signals in logarithmic mode.
Signals were amplified with the logarithmic mode for side scattering, forward scattering, and FITC fluorescence.
Forward scatter, sideward scatter, FL 1 and FL 2 were assessed using the logarithmic mode.
FITC signal was detected on one channel in the logarithmic mode, while UV fluorescence (PI) was recorded in the linear mode on a separate channel.
Ten thousand neutrophils were collected from each sample with light-scatter gain set in the linear mode and fluorescence gain set in the logarithmic mode.
Cell cycle was analyzed by measuring in the linear mode; cell death rates were determined by analyzing the fluorescence in logarithmic mode.
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