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The biggest disadvantage a biographer has with a live subject is that the biographer cannot always be as honest as he or she wants.
Seeing his work gave me the idea that I didn't need a live subject to represent a live subject.
Students are not able to communicate as effectively as they would with a live subject.
We note that tomographic FLIM in diffuse media has received considerable attention recently, particularly with respect to the development of appropriate reconstruction algorithms (see, e.g., ref. [ 48]), but tomographic FLIM of FRET in a live subject is still yet to be reported.
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It is these unsolved mysteries which make it a living subject, constantly evolving, changing and surprising.
The only time she wrote about a living subject - Margaret Thatcher, who appeared in her biographical anthology The Warrior Queens - it felt "odd".
With a living subject, "I would have a hard time writing a single page without worrying what the consequences might be... and would almost certainly end up diluting the content somewhat," Bailey observed in an e-mail message.
At the same time as capturing the challenges of trying to write about a living subject, its raison d'etre is the bizarre behaviour of the man he darkly refers to as The Other Biographer.
The case of live subject tracking is an ideal case where uncertainty theory [10] can be applied.
I don't have to live subject to the things or people I experience.
However, it is now important to study the effect on the non-invasive system of artefacts from live soft tissue in a range of live subjects.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com