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Dynamic PET data were acquired from the start of the injection for 90 min in a list mode.
Subsequently, rats were intravenously injected with 19 ± 2.0 MBq of 68Ga-DOTA-Siglec-9 via the tail vein and a 30-min PET acquisition in a list mode was performed.
A dose of 3 to 4 MBq of FDG was then injected through a tail vein, soon after the start of a list mode acquisition lasting 50 min. Acquisition was reconstructed using the following framing rate: 10 × 15 s, 5 × 30 s, 2 × 150 s, 6 × 300 s, and 1 × 600 s.
A Hoffman 3D brain phantom was filled with 20 MBq of 18F solution (FDG) at the start of scanning and scanned in a list mode or dynamic mode for 30 min together with a cylindrical phantom containing 80 MBq of 18F solution (FDG) placed on the bed 30 cm apart from the end of the phantom simulating the body activity.
At 4 weeks post-injection, mice were injected with 62.24 ± 18.90 MBq of radiotracer and underwent a dynamic SPECT session with the U-SPECT-II scanner (MILABS, Utrecht, Netherlands), using a list mode acquisition protocol consisting of 60 frames of 84 s.
A total of 15, 000 events were recorded in a list mode file and analyzed with the System II V.3 software (Beckman Coulter).
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A list-mode reconstruction based respiratory motion correction for PET has been implemented and its performance evaluated.
In addition, a list-mode system was developed to measure relatively long-lived delayed γ-rays from photofission of 238U and 239Pu after the irradiation.
The use of a list-mode data acquisition technique enabled simultaneous determination of 22Na and 7Be activity concentrations using a single measurement by coincidence and anticoincidence mode respectively.
Second, low-energy delayed γ-ray spectra (Eγ~0.6 2.7 MeV) measured with a list-mode system after irradiation of 239Pu were used in the validation.
A list-mode data file simulating dynamic PET data for the Siemens 3T MR-BrainPET was further generated, using the Utah phantom.
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