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This linkage was rapidly replicated by Rihet et al. [24] in Burkina Faso (marker D5S658) while Flori et al. [18] identified a linkage at the same marker D5S636 in a second population in Burkina Faso and an association with one marker (D5S487) within the 5q33.3 region.
Several of them described a strong relationship between depression and mortality and some could not detect a linkage at all.
While Census data have a hierarchy of geographical units, the Surveillance, Epidemiology, and End Results (SEER) data are available by county, which limited us to a linkage at the county level.
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The labeled streptavidin samples were mixed and bound to a glass coverslip through a biotin linkage at a high density, such that individual molecules were not resolvable by conventional fluorescence microscopy.
Identified by sequential linkage analysis of trait cosegregation in a full three-generation pedigree and F2 intercross mapping, a CORT-related QTL was detected on mouse chromosome 3 between 41.3 cM (closest marker: rs13477268 at 40.5 cM) and 43.3 cM (closest marker: rs4138887 at 45.3 cM) with a linkage peak at 42.3 cM.
In order to examine the effect of modifications at the 3′ overhang regions of short interfering RNAs (siRNAs) on their gene-silencing activities, we designed and synthesized novel siRNAs having thymidine dimers consisting of a carbamate or a urea linkage at their 3′ overhang regions.
All of the eQTL located outside this diagonal represent trans-linkages with a linkage peaks at loci distant from the physical location of the open reading frames.
Similarly we found a linkage signal at position 34 43 cM in chromosome 14 (NPL of 1.89), which is close to the locus (44 cM) previously reported by Smith et al. [ 15].
Briefly, 10 ng of DNA were mixed with 1.5 μL of 10× ThermoPol buffer (New England Biolabs, Ipswich, MA) and primers (random 7-mers with an additional two nitroindole residues at the 5' end and a phosphorothiate linkage at the 3' end) at a concentration of 100 μM in 15 μL.
In our intein-based and chemical schemes, we employed an intein Mxe or a thioethylbutylamido linkage at the C-terminus of their linear precursors, respectively.
In a recent study by our group [22], a SLE linkage at 13q32 was identified and replicated by restricting the samples to a relatively homogenous population of African-American families containing at least one male affected.
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