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The phosphate dolomite separation has a limited separation selectivity using conventional flotation.
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Indeed, populations 2 and 5 are very mixed; indicating only limited separation by a geographic barrier.
While LiDS-PAGE resulted in only limited separation, acidic SDS-PAGE at an actual pH of 5.5-7 (18) resembled a good compromise between separation and conservation of the acyl-phosphate linkage.
When using the FastIVA algorithm, the objective separation performance SDR and SIR are 0.19 and 2.45 dB, which confirms the limited separation performance.
Some of the delicate bifurcation structures that we identified will be more sensitive to parameter variations, but only because of their limited separation in parameter space.
The corresponding risk is particularly relevant in densely inhabited metropolitan areas, due to the usually limited separation distance between adjacent buildings.
In many laboratories, multiple, conspecific strains are often reared simultaneously with limited separation.
While this result might suggest a correlation, it should be noted that fraction 6 was the most populated SCX fraction (230 out of 693 peptides identified), and this result may simply illustrate the limited separation capabilities of SCX.
As mentioned in Section 4.1, the BSS algorithm allows to separate point sources, additional diffuse noise having only a limited impact on the separation performance [32].
This hints at a limited degree of phase separation into two amorphous phases within the blend matrix, most likely at the microscale.
In summary, these results show that the Western lineage and the ancestor of the Central and edwardsianus lineages may have been geographically isolated due to a barrier [ 41] that still may prevent mixing of the Western and edwardsianus lineages, and that niche divergence may have played a limited role in the separation of these two lineages.
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