Alternative molar ratios yield sticky and difficult to handle materials at higher polyol fractions in the reactive mixture, whilst an excess of acid terminal groups leads to a faster mass loss by hydrolysis in aqueous media together with surface salts deposition, concomitant with a lesser cell viability in in vitro culture.
The viability of primary neurons on which 0.2 µM B[a]P treated BV-2 culture supernatant was added showed a significantly reduced viability when compared to 0.02 µM treated group (p<0.01); similarly 2.0 µM group showed lesser viability than 0.2 µM treated group (p<0.01) (Fig. 6B).
Duration of surfactant incubation affected mainly incorporation of BrdU into the cells, a surrogate of DNA synthesis and cell proliferation, and to a lesser extent cellular viability.
As presented in Figure 1B, the results of the MTT assay reflect those of the PI uptake measurements: vorinostat was as effective in p53− cells as in p53+ cells, whereas entinostat had a lesser effect on the viability of p53− cells.
In contrast, MDA-MB-231 CM only increased the viability of InFs, and to a lesser extent than did integrin β4 induction.
While few HUVECs survived in PBS alone, ocular fluid maintained the viability of the cells (albeit to a lesser degree when compared to EG2).
Here, we show that p38α and to a lesser extent p38β play a key role in the viability of cancer cells treated with the chemotherapeutic agent cisplatin.
Wild-type and mutant AMPKα1 forms were overexpressed to similar degrees and without effect on cellular viability, with AMPKα2 forms expressed to a lesser degree and displaying cellular toxicity (Supplementary Material, Fig. S4).
It is also important to note that, unlike the two integrin binding antibodies, the cRGDfV peptide did alter the morphology of the tubes formed by HUVEC when sandwiched in Matrigel, although to a lesser extend than VCN and with no impact on cell viability.
A clonogenic assay also indicated relative loss of viability of MCF7-LTED cells, and, to a lesser extent, MCF7-LCC9 cells, with shRNA-mediated depletion of VAV3.
